Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53] - BSA and Azide free
Catalog# HA750630
Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53] - BSA and Azide free
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WB
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IF-Cell
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Human
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Rat
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HA721400
含抗保成分
-
unconjugated
概述
产品名称
Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Tankyrase 2 aa 451-700/1,166.
种属反应性
Human, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 127 kDa
阳性对照
PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate, PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate, A431.
偶联
unconjugated
克隆号
PSH0-53
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
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IF-Cell
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1:250
靶点
功能
Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity.
背景文献
1. Wu M et al. Down-regulation of hsa_circ_0045474 induces macrophage autophagy in tuberculosis via miR-582-5p/TNKS2 axis. Innate Immun. 2022 Jan
2. Tomassi S et al. From PARP1 to TNKS2 Inhibition: A Structure-Based Approach. ACS Med Chem Lett. 2020 Feb
亚细胞定位
Cytoplasm, Golgi apparatus membrane, Nucleus, Chromosome, telomere.
别名
ADP-ribosyltransferase diphtheria toxin-like 6 antibody
ARTD6 antibody
PARP 5b antibody
PARP 5c antibody
PARP-5b antibody
PARP-5c antibody
PARP5B antibody
PARP5C antibody
pART6 antibody
Poly [ADP ribose] polymerase 5B antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/1,000 dilution.
Lane 1: PC-12 whole cell lysate
Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate
Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Tankyrase 2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 90 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
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