Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53]

☑ Cell treatment (CT)

Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/1,000 dilution.

Lane 1: PC-12 whole cell lysate
Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate
Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 127 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Tankyrase 2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 90 kDa

Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.