Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] - BSA and Azide free
Catalog# HA750079
Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] - BSA and Azide free
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WB
-
IHC-P
-
IP
-
FC
-
IF-Tissue
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Human
概述
产品名称
Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human HO-1 aa 227-276 / 288.
种属反应性
Human
验证应用
WB, IHC-P, IP, FC, IF-Tissue
分子量
Predicted band size: 33 kDa
阳性对照
Human spleen tissue, human liver tissue, Jurkat.
偶联
unconjugated
克隆号
SP08-07
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
-
1:50-1:1,000
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FC
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1:500-1:1,000
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IP
-
Use at an assay dependent concentration.
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IF-Tissue
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1:50-1:200
靶点
功能
Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
背景文献
1. He C et al. Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration. Proc Natl Acad Sci U S A 111:14806-11 (2014).
2. Maruyama A et al. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res 42:13599-614 (2014).
序列相似性
Belongs to the heme oxygenase family.
组织特异性
Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
亚细胞定位
Endoplasmic reticulum membrane.
UNIPROT
别名
32 kD antibody
bK286B10 antibody
D8Wsu38e antibody
heat shock protein 32 kD antibody
heat shock protein 32kD antibody
Heat shock protein antibody
Heme oxygenase (decycling) 1 antibody
Heme oxygenase 1 antibody
Hemox antibody
HMOX 1 antibody
展开图片
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Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1).
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750079, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (HA750079) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750079) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (HA750079) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750079) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Heme Oxygenase 1 (HO-1)
Application:
Reactivity:
Conjugate:
