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Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

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Catalog# ET1604-45

Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

Application

  • WB

  • IHC-P

  • IP

  • FC

  • IF-Tissue

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human HO-1 aa 227-276 / 288.

种属反应性

Human

验证应用

WB, IHC-P, IP, FC, IF-Tissue

分子量

Predicted band size: 33 kDa

阳性对照

Human spleen tissue, human liver tissue, Jurkat.

偶联

unconjugated

克隆号

SP08-07

RRID

AB_3069700

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:500-1:1,000

  • IP

  • Use at an assay dependent concentration.

  • IF-Tissue

  • 1:50-1:200

发表文章中的应用

WB See 15 publications below
IHC See 1 publications below

靶点

功能

Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.

背景文献

1. He C et al. Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration. Proc Natl Acad Sci U S A 111:14806-11 (2014).

2. Maruyama A et al. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res 42:13599-614 (2014).

序列相似性

Belongs to the heme oxygenase family.

组织特异性

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

亚细胞定位

Endoplasmic reticulum membrane.

UNIPROT #

SwissProt: P09601 Human

别名

32 kD antibody

bK286B10 antibody

D8Wsu38e antibody

heat shock protein 32 kD antibody

heat shock protein 32kD antibody

Heat shock protein antibody

Heme oxygenase (decycling) 1 antibody

Heme oxygenase 1 antibody

Hemox antibody

HMOX 1 antibody

展开

图片

  • Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1).

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1604-45, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (ET1604-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1604-45" style="font-weight: bold;text-decoration: underline;">ET1604-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (ET1604-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC

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