ZO1 Recombinant Rabbit Monoclonal Antibody [PSH28-02]
Rmb: 1500
Catalog# HA724744
ZO1 Recombinant Rabbit Monoclonal Antibody [PSH28-02]
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IHC-Fr
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IF-Tissue
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IF-Cell
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FC(Intra)
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Human
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Mouse
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HA752090
不含抗保成分
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unconjugated
概述
产品名称
ZO1 Recombinant Rabbit Monoclonal Antibody [PSH28-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse ZO1 aa 1,401-1,745.
种属反应性
Human, Mouse
验证应用
IHC-Fr, IF-Tissue, IF-Cell, FC(Intra)
靶点分子量
Predicted band size: 195 kDa
阳性对照
Mouse Kidney, Human Kidney, bEnd.3 (Mouse brain microvascular endothelial cell).
偶联
unconjugated
克隆号
PSH28-02
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | FC(Intra) | IF-Cell | IF-Tissue | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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| pig |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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IF-Cell
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1:100
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FC(Intra)
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1:1,000
靶点
功能
Zonula occludens-1 ZO-1, also known as Tight junction protein-1 is a 220-kD peripheral membrane protein that is encoded by the TJP1 gene in humans. It belongs to the family of zonula occludens proteins (ZO-1, ZO-2, and ZO-3), which are tight junction-associated proteins and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions. It has a role as a scaffold protein which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.
背景文献
1. Tsurudome Y et al. Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice. Mol Biol Rep. 2022 Dec
2. Han F et al. GLTSCR1 coordinates alternative splicing and transcription elongation of ZO1 to regulate colorectal cancer progression. J Mol Cell Biol. 2022 Jun
亚细胞定位
Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome.
别名
Tight junction protein 1 antibody
Tight junction protein ZO-1 antibody
Tight junction protein ZO1 antibody
TJP1 antibody
zo-1 antibody
Zo1 antibody
ZO1_HUMAN antibody
Zona occludens 1 antibody
Zona occludens 1 protein antibody
Zona occludens protein 1 antibody
展开图片
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Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Kidney
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724744, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724744, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724744, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: bEnd.3 (Mouse brain microvascular endothelial cell) / RAW264.7 (Mouse monocytic macrophage leukemia cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724744, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Negative expression of ZO1 protein in RAW264.7 cell is consistent with the predicted expression pattern. -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) (Left, negative) / bEnd.3 (Mouse brain microvascular endothelial cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724744 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative expression of ZO1 protein in RAW264.7 cell is consistent with the predicted expression pattern.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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