CD86 Recombinant Rabbit Monoclonal Antibody [PSH25-20]
Rmb: 618 特惠 1500
Catalog# HA724514
CD86 Recombinant Rabbit Monoclonal Antibody [PSH25-20]
-
WB
-
IF-Tissue
-
IHC-P
-
Mouse
-
HA751967
不含抗保成分
-
unconjugated
概述
产品名称
CD86 Recombinant Rabbit Monoclonal Antibody [PSH25-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse CD86 aa 1-265.
种属反应性
Mouse
验证应用
WB, IF-Tissue, IHC-P
分子量
Predicted band size: 35 kDa
阳性对照
Mouse spleen tissue lysate.
偶联
unconjugated
克隆号
PSH25-20
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IF-Tissue
-
1:200
-
IHC-P
-
1:200-1:1,000
靶点
功能
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells. Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
背景文献
1. Tekguc M et al. Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells. Proc Natl Acad Sci U S A. 2021 Jul
2. Kennedy A et al. Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation. Nat Immunol. 2022 Sep
亚细胞定位
Cell membrane
UNIPROT
别名
Activation B7-2 antigen 3 antibody
Activation B7-2 antigen antibody
B-lymphocyte activation antigen B7-2 2 antibody
B-lymphocyte activation antigen B7-2 antibody
B7 2 antibody
B7 antibody
B7-2 antibody
B7.2 antibody
B70 antibody
B72 antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (HA724514) at 1/5,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1802
Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724514, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35 kDa
Observed band size: 60-85 kDa -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: Antibody A: 1/200 (CD86, HA724514, rabbit, Red), Antibody B: 1/200 (CD68, HA601596, rat, Green), overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), Goat anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724514, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724514, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724514, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号