CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]
Rmb: 880 特惠 1500
Catalog# HA724188
CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]
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IF-Cell
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FC
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Mouse
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HA751775
不含抗保成分
-
unconjugated
概述
产品名称
CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse CD38 aa 1-304.
种属反应性
Mouse
验证应用
IF-Cell, FC
靶点分子量
Predicted band size: 34 kDa
阳性对照
Mouse splenocytes.
偶联
unconjugated
克隆号
PSH20-93
反应性数据
| IF-Cell | FC | |
|---|---|---|
| Mouse |
|
|
| Human | ||
| Rat |
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Cell
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1:500
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FC
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1:1,000
靶点
功能
CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. CD38 acts as a NAD glycohydrolase in T lymphocytes. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). CD38 also plays a role in maintaining survival of an invariant NK T (iNKT) cell subset that preferentially contributes to the maintenance of immunological tolerance.
背景文献
1. Yang Q et al. NADase CD38 is a key determinant of ovarian aging. Nat Aging. 2024 Jan
2. Yu S et al. CD38-Targeting Peptide Vaccine Ameliorates Aging-Associated Phenotypes in Mice. Aging Cell. 2025 Sep
亚细胞定位
Membrane.
UNIPROT
别名
Acute lymphoblastic leukemia cells antigen CD38 antibody
ADP ribosyl cyclase 1 antibody
ADP ribosyl cyclase antibody
ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
ADP-ribosyl cyclase 1 antibody
ADPRC 1 antibody
ADPRC1 antibody
cADPr hydrolase 1 antibody
CD 38 antibody
CD38 antibody
展开图片
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Immunocytochemistry analysis of mouse splenocytes labeling CD38 with Rabbit anti-CD38 antibody (HA724188) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (HA724188) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of mouse splenocytes labeling CD38.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724188, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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