CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02]
Catalog# HA722939
CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02]
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IHC-P
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FC
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IF-Cell
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Depletion
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Mouse
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HA751202
不含抗保成分
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unconjugated
概述
产品名称
CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse CD41 aa 451-989.
种属反应性
Mouse
验证应用
IHC-P, FC, IF-Cell, Depletion
分子量
Predicted band size: 113 kDa
阳性对照
Mouse placenta tissue, mouse spleen tissue, mouse platelets.
偶联
unconjugated
克隆号
PSH08-02
反应性数据
| IHC-P | FC | IF-Cell | Depletion | |
|---|---|---|---|---|
| Mouse |
|
|
|
|
| Human |
|
|||
| Rat |
|
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IF-Cell
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1:1,000
靶点
功能
Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. ITGA2B, also known as CD41, encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. At least 38 disease-causing mutations in this gene have been discovered. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
背景文献
1. Dai L et al. Characterization of CD41(+) cells in the lymph node. Front Immunol. 2022 Aug
2. Sugimoto K et al. CD41(+) extracellular vesicles produced by avian thrombocytes contain microRNAs. Genes Cells. 2023 Dec
亚细胞定位
Membrane.
UNIPROT
别名
antigen CD41 antibody
BDPLT16 antibody
BDPLT2 antibody
CD41 antibody
CD41B antibody
form 2 antibody
GP2B antibody
GPalpha IIb antibody
GPIIb antibody
GT antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (HA722939) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722939) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (HA722939) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722939) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of mouse platelets labeling CD41.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722939, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunocytochemistry analysis of mouse platelets labeling CD41 with Rabbit anti-CD41 antibody (HA722939, cyan) at 1/1,000 dilution.
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Validation of anti-CD41 antibody depletion function in vivo.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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