Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]
Catalog# HA722288
Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]
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WB
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IHC-P
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IF-Tissue
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IP
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Mouse
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Rat
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HA751009
不含抗保成分
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unconjugated
概述
产品名称
Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Tau aa 571-620 / 758.
种属反应性
Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IP
分子量
Predicted band size: 79 kDa
阳性对照
Mouse brain tissue lysate, rat brain tissue lysate, rat kidney tissue, rat colon tissue.
偶联
unconjugated
克隆号
PSH05-50
反应性数据
| WB | IHC-P | IF-Tissue | IP | |
|---|---|---|---|---|
| Mouse |
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| Rat |
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| Human |
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产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:5,000
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IF-Tissue
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1:200-1:1,000
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IP
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1-2μg/sample
靶点
功能
The tau proteins (abbreviated from tubulin associated unit are a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). They have roles primarily in maintaining the stability of microtubules in axons and are abundant in the neurons of the central nervous system (CNS), where the cerebral cortex has the highest abundance. They are less common elsewhere but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. Pathologies and dementias of the nervous system such as Alzheimer's disease and Parkinson's disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles. The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have been characterized as intrinsically disordered proteins.
背景文献
1. Karikari TK et al. Blood phospho-tau in Alzheimer disease: analysis, interpretation, and clinical utility. Nat Rev Neurol. 2022 Jul
2. Palmqvist S et al. Prediction of future Alzheimer\'s disease dementia using plasma phospho-tau combined with other accessible measures. Nat Med. 2021 Jun
亚细胞定位
Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.
别名
AI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
展开图片
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Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (hot lysis)
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 50-70 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 79 kDa
Observed band size: 50-70 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722288 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722288 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722288 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722288 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 50 seconds; ECL: K1802
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