beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH02-83]

Western blot analysis of beta Amyloid 1-42 on different peptides with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/1,000 dilution.

Lane 1: Human Aβ37 full length peptide (negative)
Lane 2: Human Aβ38 full length peptide (negative)
Lane 3: Human Aβ39 full length peptide (negative)
Lane 4: Human Aβ40 full length peptide (negative)
Lane 5: Human Aβ41 full length peptide (negative)
Lane 6: Human Aβ42 full length peptide (positive)
Lane 7: Human Aβ43 full length peptide (negative)
Lane 8: Mouse Aβ42 full length peptide (positive)

Lysates/proteins at 100 ng/Lane.

Predicted band size: 4 kDa
Observed band size: 4 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721789) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721789) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721789) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-42 with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721789, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).