Otx2 Recombinant Rabbit Monoclonal Antibody [PSH01-61]
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Catalog# HA721704
Otx2 Recombinant Rabbit Monoclonal Antibody [PSH01-61]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
预测的种属支持售后
概述
产品名称
Otx2 Recombinant Rabbit Monoclonal Antibody [PSH01-61]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Otx2 aa 1-289.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IHC-P, IHC-Fr, IF-Tissue, IF-Cell, FC
分子量
Predicted band size: 32 kDa
阳性对照
293T transfected with FLAG-tagged Otx2 cell lysate, mouse eyeball tissue lysate, rat embryo tissue lysate, rat eyeball tissue lysate, mouse embryo tissue, mouse eye tissue, rat eye tissue, 293T overexpress with Otx2, E14.5 mouse embryo tissue.
偶联
unconjugated
克隆号
PSH01-61
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:200-1:1,000
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IHC-Fr
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1:500
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IF-Tissue
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1:200
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IF-Cell
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1:10,000
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FC
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1:1,000
靶点
功能
Homeobox protein OTX1 is a protein that in humans is encoded by the OTX1 gene. This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. The Otx gene is active in the region of the first gill arch, which is related to the upper and lower jaw and two of the bones of the ear. A similar protein in mice is required for proper brain and sensory organ development and can cause epilepsy.
背景文献
1. Zhou L et al. OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway. Oncol Rep. 2022 Nov
2. Ibad RT et al. OTX2 stimulates adult retinal ganglion cell regeneration. Neural Regen Res. 2022 Mar
亚细胞定位
Nucleus.
别名
CPHD6 antibody
Homeobox protein OTX2 antibody
MCOPS 5 antibody
MCOPS5 antibody
MGC45000 antibody
Orthodenticle 2 antibody
Orthodenticle homeobox 2 antibody
Orthodenticle homolog 2 (Drosophila) antibody
Orthodenticle homolog 2 antibody
Orthodenticle2 antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Otx2 antibody (HA721704) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721704) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Otx2 antibody (HA721704) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721704) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse eye tissue with Rabbit anti-Otx2 antibody (HA721704) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721704) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-Otx2 antibody (HA721704) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721704) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (HA721704) at 1/1,000 dilution.
Lane 1: 293T transfected with FLAG-tagged empty control cell lysate
Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 35 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721704) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (HA721704) at 1/1,000 dilution.
Lane 1: Mouse eyeball tissue lysate (40 µg/Lane)
Lane 2: Rat embryo tissue lysate (40 µg/Lane)
Lane 3: Rat eyeball tissue lysate (40 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 35 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721704) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Otx2 on Y79 cell lysates with Rabbit anti-Otx2 antibody (HA721704) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 35 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721704) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-tissue
Species: Mouse
Site: E14.5 embryo
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
☑ Cell treatment (CT)
Immunocytochemistry analysis of 293T overexpress with or without Otx2 cells labeling Otx2 with Rabbit anti-Otx2 antibody (HA721704) at 1/10,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Otx2 antibody (HA721704) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Flow cytometric analysis of 293T overexpress with or without Otx2 cells labeling Otx2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721704, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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