LI Cadherin Recombinant Rabbit Monoclonal Antibody [PD00-49]
Catalog# HA721643
LI Cadherin Recombinant Rabbit Monoclonal Antibody [PD00-49]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
概述
产品名称
LI Cadherin Recombinant Rabbit Monoclonal Antibody [PD00-49]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 92 kDa
阳性对照
Caco-2 cell lysate, LoVo cell lysate, human appendix tissue, human colon tissue, human stomach tissue.
偶联
unconjugated
克隆号
PD00-49
RRID
反应性数据
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
|
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:1,000
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IF-Tissue
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1:50-1:200
靶点
功能
Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
背景文献
1. Yui A et al. Mechanism of dimerization and structural features of human LI-cadherin. J Biol Chem. 2021 Sep
2. Wang X et al. Diagnostic value and prognostic significance of LI-cadherin and miR-378e in colorectal cancer. Oncol Lett. 2020 Sep
亚细胞定位
Cell membrane.
UNIPROT
别名
BILL-cadherin antibody
CAD17_HUMAN antibody
Cadherin 16 antibody
Cadherin 16, formerly antibody
Cadherin 17 antibody
cadherin 17, LI cadherin (liver-intestine) antibody
cadherin, liver-intestine antibody
Cadherin-17 antibody
CDH16 antibody
CDH16, formerly antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721643) at 1/5,000 dilution.
Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (negative) (20 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 120 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721643) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Stomach (Intestinal metaplasia area)
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney (Negative control)
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721643, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative control: kidney (PMID: 25768256). -
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721643) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721643, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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