TRIM25 Recombinant Rabbit Monoclonal Antibody [JE57-43]
Rmb: 699 特惠 1500
Catalog# HA721506
TRIM25 Recombinant Rabbit Monoclonal Antibody [JE57-43]
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WB
-
IF-Cell
-
IP
-
Human
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unconjugated
概述
产品名称
TRIM25 Recombinant Rabbit Monoclonal Antibody [JE57-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human TRIM25/EFP aa 100-200.
种属反应性
Human
验证应用
WB, IF-Cell, IP
靶点分子量
Predicted band size: 71 kDa
阳性对照
HepG2 cell lysate, HeLa cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, Jurkat cell lysate, 293T cell lysate, HeLa.
偶联
unconjugated
克隆号
JE57-43
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:100
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IP
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1-2μg/sample
靶点
功能
Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family grouping more than 70 TRIMs. TRIM proteins primarily function as ubiquitin ligases that regulate the innate response to infection. TRIM25 localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
背景文献
1. Liu Y et al. TRIM25 promotes the cell survival and growth of hepatocellular carcinoma through targeting Keap1-Nrf2 pathway. Nat Commun. 2020 Jan
2. Wu SF et al. RIG-I regulates myeloid differentiation by promoting TRIM25-mediated ISGylation. Proc Natl Acad Sci U S A. 2020 Jun
亚细胞定位
Cytoplasm, Stress granule, Nucleus.
UNIPROT
别名
E3 ubiquitin/ISG15 ligase TRIM25 antibody
EFP antibody
Estrogen responsive finger protein antibody
Estrogen-responsive finger protein antibody
RING finger protein 147 antibody
RNF 147 antibody
RNF147 antibody
TRI25 antibody
TRI25_HUMAN antibody
TRIM 25 antibody
展开图片
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Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (HA721506) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: A549 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721506) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (HA721506) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si TRIM25 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721506) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody (HA721506) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody (HA721506) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunoprecipitation (IP)
TRIM25 was immunoprecipitated in 0.2 mg A549 (Human lung adenocarcinoma cell) cell lysate with HA721506 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721506 at 1/,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (input)
Lane 2: HA721506 IP in A549 cell lysate
Lane 3: Rabbit IgG instead of HA721506 in A549 cell lysate
Exposure time: 6 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA721506, 1/,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 71 kDa
Observed band size: 71 kDa
引文
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HBV core protein enhances WDR46 stabilization to upregulate NUSAP1 and promote HCC progression
期刊: Hepatology Communications
DOI: 10.1097/HC9.0000000000000680
IF: 5.6
应用: WB
反应种属: Human
发表时间: 2025 May
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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