Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric) - BSA and Azide free

☑ Relative expression (RE)

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (HA601645) at 1/5,000 dilution.

Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate
Lane 3: Mouse skeletal muscle tissue lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601645, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 12 kDa
Observed band size: 12 kDa

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (HA601645) at 1/5,000 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601645, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 12 kDa
Observed band size: 12 kDa

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebellum
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA601645, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)

Species: Rat
Tissue: Cerebellum
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA601645, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601645, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1146, 20 minutes at room temperature.

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601645, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1146, 20 minutes at room temperature.

☑ Relative expression (RE)

This data was developed using HA601645, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)

Species: Human
Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.

Primary antibody: HA601645(1/1,000, Red), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 15 minutes at room temperature.

Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.