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EpCAM Recombinant Antibody [PS01-69] - Mouse IgG1 (Chimeric)

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Catalog# HA601661

EpCAM Recombinant Antibody [PS01-69] - Mouse IgG1 (Chimeric)

Application

  • IF-Cell

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

概述

产品名称

EpCAM Recombinant Antibody [PS01-69] - Mouse IgG1 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Recombinant protein within human EpCAM aa1-295.

种属反应性

Human

验证应用

IF-Cell, IHC-P

靶点分子量

Predicted band size: 35 kDa

阳性对照

HT-29, Human Endometrium, Human Colon.

偶联

unconjugated

克隆号

PS01-69

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

纯化方式

Protein A affinity purified.

应用稀释度

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:50

靶点

功能

EPCAM is a carcinoma-associated antigen and belongs to a family which includes at least 2 type I membrane proteins. The EPCAM protein has a role in embryonic stem cells proliferation and differentiation. EPCAM is used as a target for immunotherapy treatment of human carcinomas. EPCAM is expressed on most normal epithelial cells and gastrointestinal carcinomas and acts as a homotypic calcium-independent cell adhesion molecule. Epithelial cell adhesion molecules (EPCAM) can act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for supplying immunological barrier as a first line of defense against mucosal infection. EPCAM gene mutations result in congenital tufting enteropathy.

背景文献

1. Li D et al. EpCAM-targeting CAR-T cell immunotherapy is safe and efficacious for epithelial tumors. Sci Adv. 2023 Dec

2. Li F et al. EpCAM-Targeting Aptamer Radiotracer for Tumor-Specific PET Imaging. Bioconjug Chem. 2021 Jun

亚细胞定位

Lateral cell membrane, Cell junction.

UNIPROT

SwissProt: P16422 Human

别名

17 1A antibody

323/A3 antibody

Adenocarcinoma associated antigen antibody

Adenocarcinoma-associated antigen antibody

Antigen identified by monoclonal antibody

AUA1 antibody

AUA1 antibody

CD326 antibody

CD326 antigen antibody

Cell surface glycoprotein Trop 1 antibody

展开

图片

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: HT-29 (Human colorectal adenocarcinoma cell) / NCI-H1299 (Human non-small cell Lung carcinoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA601661" style="font-weight: bold;text-decoration: underline;">HA601661</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />NCI-H1299 is a low EpCAM-expressing cell line (PMID: 39105395).

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: HT-29 (Human colorectal adenocarcinoma cell) / NCI-H1299 (Human non-small cell Lung carcinoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601661, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    NCI-H1299 is a low EpCAM-expressing cell line (PMID: 39105395).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Endometrium <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601661" style="font-weight: bold;text-decoration: underline;">HA601661</a>, 1/50, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Endometrium
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601661, 1/50, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601661" style="font-weight: bold;text-decoration: underline;">HA601661</a>, 1/50, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601661, 1/50, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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