Skip to content

EpCAM Mouse Monoclonal Antibody [3-E1-E2]

RMB: 1500

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# EM1111

EpCAM Mouse Monoclonal Antibody [3-E1-E2]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

EpCAM Mouse Monoclonal Antibody [3-E1-E2]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human EP-CAM aa 24-265 (Extracellular).

种属反应性

Human, Mouse

验证应用

WB, IF-Cell

分子量

Predicted band size: 35 kDa

阳性对照

Hela cell lysate, NIH/3T3 cell lysate, MCF-7 cell lysate, D3, A431.

偶联

unconjugated

克隆号

3-E1-E2

RRID

AB_2928115

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgM

纯化方式

Protein L affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100-1:200

发表文章中的种属

Human See 4 publications below
Mouse See 3 publications below

靶点

功能

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children.

背景文献

1. "The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation." Muenz M., Kieu C., Mack B., Schmitt B., Zeidler R., Gires O. Oncogene 23:5748-5758(2004)

2. "The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7." Ladwein M., Pape U.F., Schmidt D.S., Schnoelzer M., Fiedler S., Langbein L., Franke W.W., Moldenhauer G., Zoeller M. Exp. Cell Res. 309:345-357(2005)

3. "Glycosylation is crucial for stability of tumour and cancer stem cell antigen EpCAM." Munz M., Fellinger K., Hofmann T., Schmitt B., Gires O. Front. Biosci. 13:5195-5201(2008)

4. "Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells." Ng V.Y., Ang S.N., Chan J.X., Choo A.B. Stem Cells 28:29-35(2010)

序列相似性

Belongs to the EPCAM family.

组织特异性

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.

翻译后修饰

Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

亚细胞定位

Cell membrane

UNIPROT #

SwissProt: P16422 Human

SwissProt: Q99JW5 Mouse

别名

17 1A antibody

323/A3 antibody

Adenocarcinoma associated antigen antibody

Adenocarcinoma-associated antigen antibody

Antigen identified by monoclonal antibody

AUA1 antibody

AUA1 antibody

CD326 antibody

CD326 antigen antibody

Cell surface glycoprotein Trop 1 antibody

展开

图片

  • Western blot analysis of EpCAM on different lysates using anti- EpCAM antibody at 1/1000 dilution. <br />Positive control:<br />Lane 1: Hela cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: MCF-7 cell lysate

    Western blot analysis of EpCAM on different lysates using anti- EpCAM antibody at 1/1000 dilution.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: MCF-7 cell lysate

  • ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling EpCAM with Mouse anti-EpCAM antibody (<a href="/products/EM1111" style="font-weight: bold;text-decoration: underline;">EM1111</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (<a href="/products/EM1111" style="font-weight: bold;text-decoration: underline;">EM1111</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling EpCAM with Mouse anti-EpCAM antibody (EM1111) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (EM1111) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IHC
  2. IF
  3. WB
  4. MIHC

同靶点&同通路的产品

metafield image

EpCAM Mouse Monoclonal Antibody [7E11]

Application: WB,IHC-P,ELISA

Reactivity: Human

Conjugate: unconjugated

metafield image

EpCAM Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]

Application: FC

Reactivity: Human

Conjugate: iFluor™ 647

metafield image

EpCAM Recombinant Rabbit Monoclonal Antibody [SU03-32]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]

Application: IHC-P,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单