MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric)
Rmb: 699 特惠 1500
Catalog# HA601414
MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric)
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IHC-Fr
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IHC-P
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WB
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IF-Tissue
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Human
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Mouse
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Rat
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HA610254
不含抗保成分
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within human MAG aa 1-536 / 626.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-Fr, IHC-P, WB, IF-Tissue
靶点分子量
Predicted band size: 69 kDa
阳性对照
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.
偶联
unconjugated
克隆号
PSH02-41
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | IF-Tissue | |
|---|---|---|---|---|
| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500-1:1,000
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IHC-P
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1:500
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WB
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1:2,000
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IF-Tissue
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1:500-1:1,000
靶点
功能
Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
背景文献
1. Latov N et al. Anti-MAG neuropathy: historical aspects, clinical-pathological correlations, and considerations for future therapeutical trials. Arq Neuropsiquiatr. 2024 Jun
2. Steck AJ. Anti-MAG neuropathy: From biology to clinical management. J Neuroimmunol. 2021 Dec
亚细胞定位
Cell membrane, Membrane raft.
别名
GMA antibody
MAG antibody
MAG_HUMAN antibody
Myelin associated glycoprotein antibody
Myelin-associated glycoprotein antibody
S MAG antibody
S-MAG antibody
Sialic acid binding Ig like lectin 4A antibody
Sialic acid binding immunoglobulin like lectin 4A antibody
Siglec 4a antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of MAG on different lysates with Rat anti-MAG antibody (HA601414) at 1/2,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 13 seconds; Lane 3: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
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