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Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

RMB: 699 特惠 1500

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Catalog# HA601376

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

Application

  • IHC-Fr

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Pig

  • Cynomolgus monkey

预测的种属支持售后
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within N-terminal human Iba1.

产品特异性

This Chicken Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

种属反应性

Human, Mouse, Rat (Predicted: Pig, Cynomolgus monkey)

预测的种属支持售后

验证应用

IHC-Fr, WB, FC

分子量

Predicted band size: 17 kDa

阳性对照

Mouse cerebellum tissue, mouse spleen tissue, THP-1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, THP-1.

偶联

unconjugated

克隆号

JM36-62

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgY

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

  • FC

  • 1:1,000

发表文章中的应用

IF 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下

靶点

功能

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

背景文献

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

亚细胞定位

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

UNIPROT

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

别名

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

展开

图片

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/500 (MAG, <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/500 (MAG, HA721818, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/500 (PSD95, <a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/500 (PSD95, ET1602-20, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/1,000 (F4/80, HA721520, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/1,000 (F4/80, HA721520, red)

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: HEK-293 cell lysate (negative)<br />Lane 3: Mouse spleen tissue lysate<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (HA601376) at 1/5,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: HEK-293 cell lysate (negative)
    Lane 3: Mouse spleen tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601376) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of THP-1 cells labeling Iba1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling Iba1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA601376, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IF

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