p21 Recombinant Mouse Monoclonal Antibody [A8C11-R]
概述
产品名称
p21 Recombinant Mouse Monoclonal Antibody [A8C11-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within full length of human p21.
种属反应性
Human
验证应用
IHC-P
分子量
Predicted band size: 18 kDa
阳性对照
Human thyroid cancer tissue, human cervical carcinoma tissue.
偶联
unconjugated
克隆号
A8C11-R
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
-
1:200-1:400
靶点
功能
p21WAF1, also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a protein that in humans is encoded by the CDKN1A gene located on chromosome 6 (6p21.2). May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. p21 is expressed in all adult human tissues. In tumors, the expression of p21 has been studied by immunohistochemical methods in a wide range of human tumors, such as gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. The expression of p21 is associated with favorable prognosis in various tumors.
背景文献
1. Kaya Z. et. al. CDKN1A (p21 gene) polymorphisms correlates with age in esophageal cancer. Mol Biol Rep. 2022 Jan
2. Kreis NN. et. al. The Multifaceted p21 (Cip1/Waf1/CDKN1A) in Cell Differentiation, Migration and Cancer Therapy. Cancers (Basel). 2019 Aug
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
CAP20 antibody
CDK-interacting protein 1 antibody
CDKI antibody
CDKN1 antibody
Cdkn1a antibody
CDN1A_HUMAN antibody
CIP1 antibody
Cyclin Dependent Kinase Inhibitor 1A antibody
Cyclin-dependent kinase inhibitor 1 antibody
Cyclin-dependent kinase inhibitor 1A (P21) antibody
展开图片
-
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Mouse anti-p21 antibody (HA601221) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601221) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Mouse anti-p21 antibody (HA601221) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601221) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
A Novel CSN5 Inhibitor Drives Tumor-Intrinsic PD-L1 Degradation and Exerts Direct Antitumor Efficacy in Triple-Negative Breast Cancer
期刊: Journal Of Medicinal Chemistry
DOI: 10.1021/acs.jmedchem.5c02669
IF: 6.8
应用: WB,IHC
反应种属: Human,Mouse
发表时间: 2026 Feb
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