iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
Rmb: 300
Catalog# HA1136
iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
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IF-Tissue
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IHC-Fr
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IF-Cell
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FC
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Rat
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iFluor™ 594
概述
产品名称
iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
抗体类型
Goat Polyclonal Antibody
免疫原
Rat IgG(H+L).
种属反应性
Rat
验证应用
IF-Tissue, IHC-Fr, IF-Cell, FC
偶联
iFluor™ 594
反应性数据
| IF-Tissue | IHC-Fr | IF-Cell | FC | |
|---|---|---|---|---|
| Rat |
|
|
|
|
| Human |
|
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Tissue
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1:500
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IHC-Fr
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1:500
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IF-Cell
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1:500
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FC
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1:500
靶点
功能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
背景文献
暂无
图片
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Application: IF-Tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Primary Antibody: HA601414 (1:500)
Secondary Antibody Concentration: 1: 500 -
Application: IF-Tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Primary Antibody: HA601413 (1/1,000)
Secondary Antibody Concentration: 1/500 -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Cerebellum
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601414, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 1.5 hours at room temperature. -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: C2C12 (Mouse myoblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601406, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 45 minutes at room temperature.
Counterstain: beta Tubulin (ET1602-4, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
引文
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Kinesin-related genes stratified the prognosis and immune responses of clear cell renal cell carcinoma
期刊: European Journal of Medical Research
DOI: 10.1186/s40001-025-02880-1
IF: 3.4
应用: IF-cell
反应种属: Human
发表时间: 2025 Jul
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Apelin-13 activates TFEB-mediated autophagy via AMPK to attenuate senescence and pyroptosis in nucleus pulposus cells during intervertebral disc degeneration
期刊: Mechanisms Of Ageing And Development
DOI: 10.1016/j.mad.2025.112147
IF: 5.1
应用: IF-cell
反应种属: Rat
发表时间: 2025 Dec
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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