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GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]

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Catalog# ET7107-08

GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human GABRA5 aa 1-240 / 462.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Tissue, IHC-P

分子量

Predicted band size: 52 kDa

阳性对照

A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue.

偶联

unconjugated

克隆号

JB34-19

RRID

AB_3070725

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IF-Tissue

  • 1:500

  • IHC-P

  • 1:1,000

发表文章中的应用

IHC See 2 publications below
IF See 1 publications below

发表文章中的种属

Rat See 1 publications below
rat See 1 publications below

靶点

功能

Gamma-aminobutyric acid (GABA) A receptor, alpha 5, also known as GABRA5, is a protein which in humans is encoded by the GABRA5 gene. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABAA receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABAA receptor. At least 16 distinct subunits of GABAA receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. Recent research has produced several ligands which are moderately selective for GABAA receptors containing the α5 subunit. These have proved to be useful in investigating some of the side effects of benzodiazepine and nonbenzodiazepine drugs, particularly the effects on learning and memory such as anterograde amnesia. Inverse agonists at this subunit have nootropic effects and may be useful for the treatment of cognitive disorders such as Alzheimer's disease.

背景文献

1. Denis N J et al. Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry. Proteomics 7:868-874 (2007).

2. Kaneko Y et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016).

序列相似性

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily.

亚细胞定位

Postsynaptic cell membrane, Cell membrane.

UNIPROT #

SwissProt: P31644 Human

SwissProt: Q8BHJ7 Mouse

SwissProt: P19969 Rat

别名

GAA 5 antibody

GAA5 antibody

GABA(A) receptor subunit alpha-5 antibody

GABRA 5 antibody

Gabra5 antibody

Gamma aminobutyric acid GABA A receptor alpha 5 antibody

Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody

Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody

Gamma-aminobutyric acid receptor subunit alpha-5 antibody

GBRA5_HUMAN antibody

展开

图片

  • Western blot analysis of GABRA5 on different lysates with Rabbit anti-GABRA5 antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 52 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GABRA5 on different lysates with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/2,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Neuro-2a cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 52 kDa
    Observed band size: 70 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABRA5 antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABRA5 antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABRA5 with Rabbit anti-GABRA5 antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET7107-08" style="font-weight: bold;text-decoration: underline;">ET7107-08</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABRA5 with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7107-08, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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