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VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82]

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Catalog# ET7107-01

VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human VPS35 aa 80-220 / 796.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

分子量

Predicted band size: 92 kDa

阳性对照

U-937 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse colon tissue lysate, L6 cell lysate, rat colon tissue lysate, human colon cancer tissue, human kidney tissue, human placenta tissue, mouse colon tissue.

偶联

unconjugated

克隆号

JB33-82

RRID

AB_3070718

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:100-1:300

靶点

功能

Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes.

背景文献

1. Arighi C N et al. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol 165:123-133 (2004) .

2. Verges M et al. The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor. Nat Cell Biol 6:763-769 (2004).

序列相似性

Belongs to the VPS35 family.

组织特异性

Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.

亚细胞定位

Cytoplasm. Membrane. Endosome.

UNIPROT #

SwissProt: Q96QK1 Human

SwissProt: Q9EQH3 Mouse

Entrez Gene: 25479 Rat

别名

DKFZp434E1211 antibody

DKFZp434P1672 antibody

FLJ10752 antibody

FLJ13588 antibody

FLJ20388 antibody

hVPS35 antibody

Maternal embryonic 3 antibody

Maternal-embryonic 3 antibody

MEM 3 antibody

MEM3 antibody

展开

图片

  • Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/1,000 dilution.<br /><br />Lane 1: U-937 cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: C2C12 cell lysate (20 µg/Lane)<br />Lane 5: Mouse colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (ET7107-01) at 1/1,000 dilution.

    Lane 1: U-937 cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: C2C12 cell lysate (20 µg/Lane)
    Lane 5: Mouse colon tissue lysate (40 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 80 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-01) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-VPS35 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (ET7107-01) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-VPS35 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 80 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-01) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/1,000 dilution.<br /><br />Lane 1: L6 cell lysate (10 µg/Lane)<br />Lane 2: Rat colon tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 24 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-01" style="font-weight: bold;text-decoration: underline;">ET7107-01</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (ET7107-01) at 1/1,000 dilution.

    Lane 1: L6 cell lysate (10 µg/Lane)
    Lane 2: Rat colon tissue lysate (20 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 80 kDa

    Exposure time: 24 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-01) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"