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Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953]

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Catalog# ET1702-04

Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human CD206 aa 1,407-1,456 / 1,456.

种属反应性

Human, Mouse

验证应用

WB, IF-Cell, FC

分子量

Predicted band size: 166 kDa

阳性对照

MOLT-4 cell lysate, Mouse lung tissue lysate, Human lung tissue lysate, HepG2 cell lysate, D3 cell lysate, HepG2.

偶联

unconjugated

克隆号

JF0953

RRID

AB_2893392

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:100

  • FC

  • 1:500-1:1,000

靶点

功能

CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. It is an phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.

背景文献

1. Huen SC et al. GM-CSF Promotes Macrophage Alternative Activation after Renal Ischemia/Reperfusion Injury. J Am Soc Nephrol 26:1334-45 (2015).

2. Lean QY et al. Orally Administered Enoxaparin Ameliorates Acute Colitis by Reducing Macrophage-Associated Inflammatory Responses. PLoS One 10:e0134259 (2015).

亚细胞定位

Endosome membrane, Cell membrane.

UNIPROT

SwissProt: P22897 Human

SwissProt: Q61830 Mouse

别名

bA541I19.1 antibody

C type lectin domain family 13 member D antibody

C-type lectin domain family 13 member D antibody

CD 206 antibody

CD206 antibody

CD206 antigen antibody

CLEC13D antibody

CLEC13DL antibody

Macrophage mannose receptor 1 antibody

Macrophage mannose receptor 1 like protein 1 antibody

展开

图片

  • Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>) at 1/5,000 dilution.<br /><br />Lane 1: MOLT-4 cell lysate (20 µg/Lane)<br />Lane 2: Mouse lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 166 kDa<br />Observed band size: 166 kDa<br /><br />Exposure time: 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/5,000 dilution.

    Lane 1: MOLT-4 cell lysate (20 µg/Lane)
    Lane 2: Mouse lung tissue lysate (40 µg/Lane)

    Predicted band size: 166 kDa
    Observed band size: 166 kDa

    Exposure time: 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-04) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />All lanes: Western blot analysis of CD206 with anti-CD206 antibody[JF0953] (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>) at 1:1,000 dilution.<br />Lane 1: Wild-type HepG2 whole cell lysate (10 µg).<br />Lane 2: CD206 knockdown HepG2 whole cell lysate (10 µg).<br />Lane 3: CD206 knockdown HepG2 whole cell lysate (10 µg).<br /><br /><a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a> was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    All lanes: Western blot analysis of CD206 with anti-CD206 antibody[JF0953] (ET1702-04) at 1:1,000 dilution.
    Lane 1: Wild-type HepG2 whole cell lysate (10 µg).
    Lane 2: CD206 knockdown HepG2 whole cell lysate (10 µg).
    Lane 3: CD206 knockdown HepG2 whole cell lysate (10 µg).

    ET1702-04 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-04, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Human lung tissue lysate<br />Lane 2: HepG2 cell lysate

    Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Human lung tissue lysate
    Lane 2: HepG2 cell lysate

  • Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse lung tissue lysate<br />Lane 2: D3 cell lysate

    Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse lung tissue lysate
    Lane 2: D3 cell lysate

  • Immunocytochemistry analysis of HepG2 cells labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mannose Receptor(CD206) antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HepG2 cells labeling Mannose Receptor(CD206).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1702-04" style="font-weight: bold;text-decoration: underline;">ET1702-04</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling Mannose Receptor(CD206).

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-04, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

同靶点 & 同通路的产品

metafield image

Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953] - BSA and Azide free

Application: WB,IF-Cell,FC

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

FITC Conjugated Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953]

Application: FC

Reactivity: Human

Conjugate: FITC

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