Human/Monkey Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-46] - BSA and Azide free (Capture)
Catalog# HA724377
Human/Monkey Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-46] - BSA and Azide free (Capture)
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ELISA(Cap)
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Human
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Monkey
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15.6-4,000 pg/mL
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7.18
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unconjugated
概述
产品名称
Human/Monkey Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-46] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Mannose Receptor(CD206) aa 1-361 (HA211390).
种属反应性
Human, Monkey
验证应用
ELISA(Cap)
阳性对照
Recombinant Human Mannose Receptor(CD206) protein (HA211390).
偶联
unconjugated
克隆号
PSH23-46
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-47] to Human/Monkey Mannose Receptor(CD206) (Detecor) (HA724378) and Recombinant Human Mannose Receptor(CD206) protein (HA211390) as the standard. The reference range value is 15.6-4,000 pg/mL.
靶点
功能
CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. It is an phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.
背景文献
1. Ray A et al. Targeting CD206+ macrophages disrupts the establishment of a key antitumor immune axis. J Exp Med. 2025 Jan
2. Pommerolle L et al. CD206(+) macrophages are relevant non-invasive imaging biomarkers and therapeutic targets in experimental lung fibrosis. Thorax. 2024 Nov
亚细胞定位
Endosome membrane, Cell membrane.
UNIPROT
别名
bA541I19.1 antibody
C type lectin domain family 13 member D antibody
C-type lectin domain family 13 member D antibody
CD 206 antibody
CD206 antibody
CD206 antigen antibody
CLEC13D antibody
CLEC13DL antibody
Macrophage mannose receptor 1 antibody
Macrophage mannose receptor 1 like protein 1 antibody
展开图片
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Sandwich ELISA analysis of Human/Monkey Mannose Receptor(CD206) matched pair antibodies
Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724377) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Mannose Receptor(CD206) protein (HA211390) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native Mannose Receptor(CD206) in human serum samples.
Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]
The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curve and corrected for sample dilution. Undiluted samples are human serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mannose Receptor(CD206) concentration was determined to be 159.7 ng/ml in human serum. -
Interpolated concentrations of native Mannose Receptor(CD206) in Cynomolgus serum samples.
Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]
The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curve and corrected for sample dilution. Undiluted samples are Cynomolgus serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mannose Receptor(CD206) concentration was determined to be 116.1 ng/ml in Cynomolgus serum. -
Interpolated concentrations of spiked Mannose Receptor(CD206) in human cell culture media samples.
Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]
The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953]
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Biotin Conjugated Human/Monkey Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-47] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human,Monkey
Conjugate: Biotin
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