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HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]

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Catalog# ET1611-49

HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.

种属反应性

Species independent

验证应用

WB, IP

阳性对照

N-terminal HA-tagged recombinant protein, C-terminal HA-tagged recombinant protein.

偶联

unconjugated

克隆号

SN07-06

RRID

AB_3070028

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IP

  • 2-5 µg/ml.

靶点

功能

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA tag recombinant protein.

背景文献

1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).

2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).

别名

HA epitope tag antibody

HA1 antibody

HA2 antibody

hemagglutinin antibody

Hemagglutinin HA1 chain antibody

Hemagglutinin HA2 chain antibody

图片

  • Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (<a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a>) at 1/5,000 dilution.<br /><br />Lane 1: N-terminal HA-tagged recombinant protein<br />Lane 2: C-terminal HA-tagged recombinant protein<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a>, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa

    Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (ET1611-49) at 1/5,000 dilution.

    Lane 1: N-terminal HA-tagged recombinant protein
    Lane 2: C-terminal HA-tagged recombinant protein

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1611-49, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with <a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/M1008-1" style="font-weight: bold;text-decoration: underline;">M1008-1</a> at 1/5,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: HA Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a> IP in HA Tag fusion protein lysate.<br />Lane 3: <a href="/products/0906-1" style="font-weight: bold;text-decoration: underline;">0906-1</a> IP in HA Tag fusion protein lysate.<br />Lane 4: Rabbit IgG instead of <a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a> in HA Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/5,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.

    Lane 1: HA Tag fusion protein lysate (input).
    Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
    Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
    Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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