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ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02]

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Catalog# ET1605-24

ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IP

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

  • Cynomolgus monkey

  • Pig

预测的种属支持售后
This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human ALDH1A1 aa 300-360.

种属反应性

Human, Mouse (Predicted: Rat, Cynomolgus monkey, Pig)

预测的种属支持售后

验证应用

WB, IHC-P, IHC-Fr, IP

分子量

Predicted band size: 55 kDa

阳性对照

A549 cell lysate, mouse lung tissue lysate, human liver tissue lysate, human kidney tissue lysate, human liver tissue, human kidney tissue, mouse striatum tissue.

偶联

unconjugated

克隆号

SY11-02

RRID

AB_3069708

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:100-1:200

  • IHC-Fr

  • 1:200

  • IP

  • Use at an assay dependent concentration.

发表文章中的应用

WB 查看 2 篇文献如下
IHC-P 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 3 篇文献如下
Human 查看 1 篇文献如下

靶点

功能

Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during the detoxification of alcohol-derived acetaldehyde; metabolism of corticosteroids, biogenic amines and neurotransmitters; and lipid peroxidation. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1), aldehyde dehydrogenase family 1 member A1, aldehyde dehydrogenase cytosolic, ALDHII, ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). There are two major liver isoforms of ALDH1 that can localize to cytosolic or mitochondrial space. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH1A3 (ALDH6, RALDH3, ALDH1A6) is a 37 kb gene that consists of 13 exons and produces a major transcript of approximately 3.5 kb most abundant in salivary gland, stomach and kidney. ALDH3A1 (stomach type, ALDH3, ALDHIII) forms a cytoplasmic homodimer that preferentially oxidizes aromatic aldehyde substrates. ALDH genes upregulate as a part of the oxidative stress response, and appear to be abundant in certain tumors that have an accelerated metabolism toward chemotherapy agents.

背景文献

1. Lokody IB et al. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS One 10:e0129470 (2015).

2. Foster JW et al. Low-glucose enhances keratocyte-characteristic phenotype from corneal stromal cells in serum-free conditions. Sci Rep 5:10839 (2015).

序列相似性

Belongs to the aldehyde dehydrogenase family.

翻译后修饰

The N-terminus is blocked most probably by acetylation.

亚细胞定位

Cytoplasm,Cell projection.

UNIPROT

SwissProt: P00352 Human

SwissProt: P24549 Mouse

SwissProt: P51647 Rat

别名

Acetaldehyde dehydrogenase 1 antibody

AHD2 antibody

AL1A1_HUMAN antibody

ALDC antibody

Aldehyde dehydrogenase 1 family member A1 antibody

Aldehyde dehydrogenase 1 soluble antibody

Aldehyde dehydrogenase 1A1 antibody

Aldehyde dehydrogenase antibody

Aldehyde dehydrogenase cytosolic antibody

Aldehyde dehydrogenase family 1 member A1 antibody

展开

图片

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD ALDH1A1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 5 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/2,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD ALDH1A1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 50 kDa

    Exposure time: 5 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Striatum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:200<br /><br />Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: Striatum

    Sample: Frozen section

    Antibody concentration: 1:200

    Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-ALDH1A1 antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/2,000 dilution.<br /><br />Lane 1: mouse lung tissue lysate<br />Lane 2: human liver tissue lysate<br />Lane 3: human kidney tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/2,000 dilution.

    Lane 1: mouse lung tissue lysate
    Lane 2: human liver tissue lysate
    Lane 3: human kidney tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1605-24" style="font-weight: bold;text-decoration: underline;">ET1605-24</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P

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