ALDH1A1 Mouse Monoclonal Antibody [8F1]

Western blot analysis of ALDH1A1 on different lysates with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Human lung tissue lysate (40 µg/Lane)

Predicted band size: 55 kDa
Observed band size: 50 kDa

Exposure time: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of ALDH1A1 on different lysates with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/2,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD ALDH1A1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 50 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A549 cells labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.

Flow cytometric analysis of A549 cells labeling ALDH1A1.

Cells were fixed and permeabilized. Then stained with the primary antibody (EM1801-07, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Immunofluorescence analysis of frozen mouse substantia nigra tissue labeling ALDH1A1 with Mouse anti-ALDH1A1 antibody (EM1801-07).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (EM1801-07, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.