GFP Recombinant Rabbit Monoclonal Antibody [SP069-0]
Catalog# ET1604-26
GFP Recombinant Rabbit Monoclonal Antibody [SP069-0]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Species independent
概述
产品名称
GFP Recombinant Rabbit Monoclonal Antibody [SP069-0]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein of Aequorea victoria GFP.
种属反应性
Species independent
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 27 kDa
阳性对照
K562 cell lysates transfected with GFP.
偶联
unconjugated
克隆号
SP069-0
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:10,000
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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IHC-P
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1:1,000-1:5,000
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IP
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2-5 µg/ml.
发表文章中的应用
发表文章中的种属
| Mouse | See 1 publications below |
靶点
功能
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
背景文献
1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.
2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.
UNIPROT #
别名
GFP antibody
Green fluorescent protein antibody
yfp antibody
图片
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Western blot analysis of GFP on K562 cell lysates transfected with GFP.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-26, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. -
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1604-26 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: Rabbit IgG instead of ET1604-26 in GFP Tag fusion protein lysate.
Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1604-26) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-26) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1604-26) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-26) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma
Author: Lijuan Chen,Yiguo Hu
PMID: 31666930
应用: WB
反应种属: Mouse
发表时间: 2019 Oct
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Citation