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GFP Mouse Monoclonal Antibody [B2-F7-C10]

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Catalog# M1004-8

GFP Mouse Monoclonal Antibody [B2-F7-C10]

Application

  • WB

Reactivity

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GFP Mouse Monoclonal Antibody [B2-F7-C10]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant full length protein of Aequorea victoria GFP.

种属反应性

Species independent

验证应用

WB

分子量

26 kDa

阳性对照

K562 cell lysates transfected with GFP.

偶联

unconjugated

克隆号

B2-F7-C10

RRID

AB_3073013

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein G affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

发表文章中的应用

IF See 1 publications below

发表文章中的种属

Species independent See 1 publications below

靶点

功能

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

背景文献

1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.

2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.

UNIPROT #

SwissProt: P42212 AequoreaVictoria

别名

GFP antibody

Green fluorescent protein antibody

图片

  • Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M1004-8" style="font-weight: bold;text-decoration: underline;">M1004-8</a>, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1004-8, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IF