ROCK1 Rabbit Polyclonal Antibody
Catalog# ER40122
ROCK1 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
-
Mouse
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Rat
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unconjugated
概述
产品名称
ROCK1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within residues of ROCK1 aa 1,305-1,354 / 1,354.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 158 kDa
阳性对照
A431 cell lysate, Human lung tissue lysate, Mouse spleen tissue lysate, C6 cell lysate, L6 cell lysate, HAP1 cell lysate, human colon cancer tissue, rat brain tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
-
1:1,000-1:2,000
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IHC-P
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1:200
靶点
功能
ROCK1 is a protein serine/threonine kinase also known as rho-associated, coiled-coil-containing protein kinase 1. ROCK1 has a ubiquitous tissue distribution, but subcellularly it is thought to colocalize with the centrosomes. This is consistent with its function as a key modulator of cell motility, tumor cell invasion, and actin cytoskeleton organization. ROCK1 has a diverse range of functions in the body. It is a key regulator of actin-myosin contraction, stability, and cell polarity. These contribute to many progresses such as regulation of morphology, gene transcription, proliferation, differentiation, apoptosis and oncogenic transformation. Other functions involve smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility. These functions are activated by phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A.
背景文献
1. "Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage." Ongusaha P.P., Qi H.H., Raj L., Kim Y.B., Aaronson S.A., Davis R.J., Shi Y., Liao J.K., Lee S.W. Sci. Signal. 1:RA14-RA14(2008)
2. "Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation." Lock F.E., Hotchin N.A. PLoS ONE 4:E8190-E8190(2009)
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
组织特异性
Detected in blood platelets.
翻译后修饰
Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
亚细胞定位
Cytoplasm, cell membrane, Nucleus, Cell projection, Cytoskeleton, Golgi apparatus, Membrane.
别名
coiled-coil-containing protein kinase 1 antibody
coiled-coil-containing protein kinase I antibody
MGC131603 antibody
MGC43611 antibody
p160 Rhoassociated coiled coil-forming protein kinase antibody
p160 ROCK-1 antibody
p160 ROCK1 antibody
p160ROCK antibody
PRO0435 antibody
Renal carcinoma antigen NY REN 35 antibody
展开图片
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Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: C6 cell lysate
Lane 5: L6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ROCK1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19]
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点 & 同通路的产品
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19]
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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