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Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

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Catalog# ET1603-1

Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Caveolin-1 aa 129-178 / 178.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IF-Tissue, IHC-P

分子量

Predicted band size: 20 kDa

阳性对照

A549 cell lysate, A431 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse heart tissue lysate, rat lung tissue lysate, rat heart tissue lysate, A549, MCF-7, NIH/3T3, human liver tissue, human liver carcinoma tissue, human uterus tissue, mouse heart tissue.

偶联

unconjugated

克隆号

SZ02-01

RRID

AB_3069667

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:1,000

  • IHC-P

  • 1:200-1:10,000

靶点

功能

Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.

背景文献

1. Chen D et al. Glioma cell proliferation controlled by ERK activity-dependent surface expression of PDGFRA. PLoS One 9:e87281 (2014).

2. Olofsson A et al. Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways. MBio 5:e00979-14 (2014).

序列相似性

Belongs to the caveolin family.

组织特异性

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

翻译后修饰

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

亚细胞定位

Golgi apparatus membrane, Cell membrane, Membrane.

UNIPROT

SwissProt: Q03135 Human

SwissProt: P49817 Mouse

SwissProt: P41350 Rat

别名

BSCL3 antibody

CAV antibody

CAV1 antibody

CAV1_HUMAN antibody

caveolae protein, 22 kD antibody

caveolin 1 alpha isoform antibody

caveolin 1 beta isoform antibody

Caveolin 1 caveolae protein 22kDa antibody

Caveolin-1 antibody

Caveolin1 antibody

展开

图片

  • Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at different dilutions.<br /><br />Lane 1/2: A549 cell lysate<br />Lane 3/4: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at different dilutions.

    Lane 1/2: A549 cell lysate
    Lane 3/4: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (10 µg/Lane)<br />Lane 2: A431 cell lysate (10 µg/Lane)<br />Lane 3: Human lung tissue lysate (40 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat lung tissue lysate (40 µg/Lane)<br />Lane 7: Rat heart tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20/18 kDa<br /><br />Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (10 µg/Lane)
    Lane 2: A431 cell lysate (10 µg/Lane)
    Lane 3: Human lung tissue lysate (40 µg/Lane)
    Lane 4: Mouse lung tissue lysate (40 µg/Lane)
    Lane 5: Mouse heart tissue lysate (40 µg/Lane)
    Lane 6: Rat lung tissue lysate (40 µg/Lane)
    Lane 7: Rat heart tissue lysate (40 µg/Lane)

    Predicted band size: 20 kDa
    Observed band size: 20/18 kDa

    Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Caveolin-1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Caveolin-1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Caveolin-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Caveolin-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Caveolin-1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Caveolin-1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-1) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: heart<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Mouse

    Site: heart

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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