iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
Catalog# HA720220F
iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
-
IF-Tissue
-
Human
概述
产品名称
iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD31 aa 689-738 / 738.
种属反应性
Human
验证应用
IF-Tissue
分子量
Predicted band size: 83 kDa
阳性对照
Human tonsils tissue.
偶联
iFluor™ 488
克隆号
SU03-59
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
-
1:200
靶点
功能
PECAM-1 is a cell-cell adhesion protein which interacts with other PECAM-1 molecules through homophilic interactions or with non-PECAM-1 molecules through heterophilic interactions. Homophilic interactions between PECAM-1 molecules are mediated by antiparallel interactions between extracellular Ig-like domain 1 and Ig-like domain 2. These interactions are regulated by the level of PECAM-1 expression. Homophilic interactions occur, only when the surface expression of PECAM-1 is high. Otherwise, when expression is low, heterophilic interactions occur.
背景文献
1. Doi H et al. Potency of umbilical cord blood- and Wharton\'s jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep 6:18844 (2016).
2. Yang Y et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015).
亚细胞定位
Cell junction. Cell membrane. Membrane.
UNIPROT #
别名
Adhesion molecule antibody
CD31 antibody
CD31 antigen antibody
CD31 EndoCAM antibody
EndoCAM antibody
FLJ34100 antibody
FLJ58394 antibody
GPIIA antibody
GPIIA' antibody
PECA1 antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded human tonsils tissue labeling CD31 with Rabbit anti-CD31 antibody (HA720220F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720220F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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