iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
Rmb: 3280
Catalog# HA720220F
iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
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IF-Tissue
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Human
-
iFluor™ 488
概述
产品名称
iFluor™ 488 Conjugated CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD31 aa 689-738 / 738.
种属反应性
Human
验证应用
IF-Tissue
分子量
Predicted band size: 83 kDa
阳性对照
Human tonsils tissue.
偶联
iFluor™ 488
克隆号
SU03-59
反应性数据
| IF-Tissue | |
|---|---|
| Human |
|
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
-
1:200
靶点
功能
PECAM-1 is a cell-cell adhesion protein which interacts with other PECAM-1 molecules through homophilic interactions or with non-PECAM-1 molecules through heterophilic interactions. Homophilic interactions between PECAM-1 molecules are mediated by antiparallel interactions between extracellular Ig-like domain 1 and Ig-like domain 2. These interactions are regulated by the level of PECAM-1 expression. Homophilic interactions occur, only when the surface expression of PECAM-1 is high. Otherwise, when expression is low, heterophilic interactions occur.
背景文献
1. Doi H et al. Potency of umbilical cord blood- and Wharton\'s jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep 6:18844 (2016).
2. Yang Y et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015).
亚细胞定位
Cell junction. Cell membrane. Membrane.
UNIPROT
别名
Adhesion molecule antibody
CD31 antibody
CD31 antigen antibody
CD31 EndoCAM antibody
EndoCAM antibody
FLJ34100 antibody
FLJ58394 antibody
GPIIA antibody
GPIIA' antibody
PECA1 antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded human tonsils tissue labeling CD31 with Rabbit anti-CD31 antibody (HA720220F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720220F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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