CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)
Catalog# HA601605
CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)
-
WB
-
IHC-P
-
Human
-
HA610405
不含抗保成分
-
unconjugated
概述
产品名称
CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human CD21 aa 1000 to the C-terminus.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 113 kDa
偶联
unconjugated
克隆号
PD00-23
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
-
1:4,000
靶点
功能
CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CD21 plays an important role in enhancing mature B cell responses to foreign antigens.
背景文献
1. Smith NA. et. al. CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. J Virol. 2020 May
2. Visentini M. et. al. CD21(low) B cells are predictive markers of new digital ulcers in systemic sclerosis. Clin Exp Immunol. 2021 Aug
亚细胞定位
Cell membrane.
UNIPROT
别名
C3DR antibody
CD 21 antibody
CD21 antibody
Complement C3d receptor 2 antibody
Complement C3d receptor antibody
Complement component (3d/Epstein Barr virus) receptor 2 antibody
Complement receptor type 2 antibody
CR antibody
Cr2 antibody
CR2_HUMAN antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (HA601605) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cells)
Lane 2: K-562 (Human chronic myelogenous leukemia cells)
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601605, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 113 kDa
Observed band size: 150 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lymph node
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601605, 1/4,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601605, 1/4,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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