p16INK4a Mouse Monoclonal Antibody [A4A5]
Catalog# HA600026
p16INK4a Mouse Monoclonal Antibody [A4A5]
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WB
-
IHC-P
-
Human
概述
产品名称
p16INK4a Mouse Monoclonal Antibody [A4A5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human p16INK4a aa 1-156.
种属反应性
Human
验证应用
WB, IHC-P
分子量
16 kDa
阳性对照
293 cell lysate, Hela cell lysate, human ovarian serous carcinoma tissue, human cervical poorly differentiated adenocarcinoma tissue.
偶联
unconjugated
克隆号
A4A5
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
-
1:500
-
IHC-P
-
1:100-1:500
靶点
功能
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
背景文献
1. Okamoto A. et. al. Mutations and altered expression of p16INK4 in human cancer. Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994).
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
ARF antibody
CDK4 inhibitor p16 INK4 antibody
CDK4I antibody
CDKN2 antibody
CDKN2A antibody
Cell cycle negative regulator beta antibody
CMM2 antibody
Cyclin dependent kinase 4 inhibitor A antibody
Cyclin dependent kinase inhibitor 2A antibody
INK4 antibody
展开图片
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Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Hela cell lysate -
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600026, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human cervical poorly differentiated adenocarcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600026, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
p16INK4a Mouse Monoclonal Antibody [A4A3]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
