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p16INK4a Recombinant Rabbit Monoclonal Antibody [SU0702]

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Catalog# ET1608-62

p16INK4a Recombinant Rabbit Monoclonal Antibody [SU0702]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

p16INK4a Recombinant Rabbit Monoclonal Antibody [SU0702]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human p16INK4a aa 107-156 / 156.

种属反应性

Human

验证应用

WB, IP

分子量

Predicted band size: 16 kDa

阳性对照

293 cell lysate, SiHa cell lysate, HepG2, Hela, PC-3M, human colon carcinoma tissue, human cervical carcinoma tissue, human ovary carcinoma tissue.

偶联

unconjugated

克隆号

SU0702

RRID

AB_3069819

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

发表文章中的应用

WB 查看 7 篇文献如下

靶点

功能

p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. It is encoded by the CDKN2A gene. A deletion (the omission of a part of the DNA sequence during replication) in this gene can result in insufficient or non-functional p16, accelerating the cell cycle and resulting in many types of cancer. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer.

背景文献

1. Idriss MH et al. Orthokeratotic Bowen disease: a histopathologic, immunohistochemical and molecular study. J Cutan Pathol 43:24-31 (2016).

2. Liang L et al. Assessment of the Utility of PAX8 Immunohistochemical Stain in Diagnosing Endocervical Glandular Lesions. Arch Pathol Lab Med 140:148-52 (2016).

序列相似性

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

组织特异性

Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

翻译后修饰

Phosphorylation seems to increase interaction with CDK4.

亚细胞定位

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P42771 Human

别名

CCM2 antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN2 antibody

CDKN2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody

Cyclin Dependent Kinase Inhibitor 2A antibody

展开

图片

  • Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-62" style="font-weight: bold;text-decoration: underline;">ET1608-62</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: 293 cell lysate<br />Lane 2: SiHa cell lysate

    Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-62, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 293 cell lysate
    Lane 2: SiHa cell lysate

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (<a href="/products/ET1608-62" style="font-weight: bold;text-decoration: underline;">ET1608-62</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si p16INK4a cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-62" style="font-weight: bold;text-decoration: underline;">ET1608-62</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (ET1608-62) at 1/1,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si p16INK4a cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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