mCherry Rabbit Polyclonal Antibody
Catalog# HA500049
mCherry Rabbit Polyclonal Antibody
-
WB
-
ELISA
-
IF-Cell
-
Species independent
概述
产品名称
mCherry Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within mCherry 1-236aa.
种属反应性
Species independent
验证应用
WB, ELISA, IF-Cell
阳性对照
Recombinant mCherry protein lysates.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:5,000
-
ELISA
-
1:1,000-1:5,000
-
IF-Cell
-
1:10,000
发表文章中的应用
发表文章中的种属
| Human | See 1 publications below |
靶点
功能
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. Genome editing has been improved greatly through the precise insertion of these fluorescent protein tags into the genetic material of many diverse organisms. Most comparisons between the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that affect protein performance in cells or organisms. It is hard to perfectly simulate cellular environments in vitro, and the difference in environment could have an effect on the brightness and photostability. This product recognizes tdTomato.
背景文献
1. Shaner, Nathan C,.et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology. 22 (12): 1567–1572.
2. Shu, Xiaokun,. et al. Novel Chromophores and Buried Charges Control Color in mFruits†,‡. Biochemistry. 45 (32): 9639–9647.
UNIPROT #
图片
-
Western blot analysis of mCherry on different lysates with Rabbit anti-mCherry antibody (HA500049) at 1/5,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with mCherry cell lysate
Lysates/proteins at 5 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500049) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ELISA analysis of with anti-mCherry antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-mCherry antibody was used as the capture antigen for ELISA.
-
Immunocytochemistry analysis of HeLa cells transfected with or without mCherry labeling mCherry with Rabbit anti-mCherry antibody (HA500049) at 1/10,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mCherry antibody (HA500049) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
Alternative Products
