c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Application: IHC-Fr

Species: Mouse

Site: Cerebral cortex (restraint stress induced)

Sample: Frozen section

Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green)

Antigen retrieval: Not required

Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

Application: IHC-Fr

Species: Rat

Site: Cerebral cortex (restraint stress induced)

Sample: Frozen section

Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green)

Antigen retrieval: Not required

Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

☑ Cell treatment (CT)

Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (HA610220) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
Lysates/proteins at 15 µg/Lane.

Predicted band size: 41 kDa
Observed band size: 41-55 kDa
Exposure time: 6 seconds; ECL: K1801;

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610220) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/10,000 dilution was used for 1 hour at room temperature.