WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-43]
Catalog# HA723749
WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-43]
-
WB
-
IF-Cell
-
IP
-
Human
-
Mouse
-
Rat
-
Monkey
-
HA751558
不含抗保成分
-
unconjugated
概述
产品名称
WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human WWOX aa 1-414.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB, IF-Cell, IP
分子量
Predicted band size: 47 kDa
阳性对照
MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Rat brain tissue lysate, NIH/3T3.
偶联
unconjugated
克隆号
PSH15-43
反应性数据
| WB | IF-Cell | IP | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
|
| Monkey |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:10,000
-
IF-Cell
-
1:100
-
IP
-
1-2μg/sample
靶点
功能
WW domain-containing oxidoreductase is an enzyme that in humans is encoded by the WWOX gene. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms. WWOX is also known as human accelerated region 6. It may, therefore, have played a key role in differentiating humans from apes.
背景文献
1. Hsu CY et al. WWOX and Its Binding Proteins in Neurodegeneration. Cells. 2021 Jul
2. Baryła I et al. WWOX and metabolic regulation in normal and pathological conditions. J Mol Med (Berl). 2022 Dec
亚细胞定位
Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus, Lysosome.
别名
5330426P09Rik antibody
9030416C10Rik antibody
Aberrant WW domain-containing oxidoreductase antibody
D16S432E antibody
EC 1.1.1.- antibody
EIEE28 antibody
FOR antibody
FRA16D antibody
Fragile site FRA16D oxidoreductase antibody
Fragile site FRA16D Oxireductase antibody
展开图片
-
Western blot analysis of WWOX on different lysates with Rabbit anti-WWOX antibody (HA723749) at 1/10,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: COS-1 cell lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723749) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling WWOX with Rabbit anti-WWOX antibody (HA723749) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WWOX antibody (HA723749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
WWOX was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA723749 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723749 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (input)
Lane 2: HA723749 IP in MCF7 cell lysate
Lane 3: Rabbit IgG instead of HA723749 in MCF7 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-43] - BSA and Azide free
Application: WB,IF-Cell,IP
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
WWOX Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-44]
Application: WB,IP
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
浙公网安备 33019202000643号