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V5 tag Mouse Monoclonal Antibody [A10-D0-A1]

RMB: 599 特惠 1200

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Catalog# M1008-2

V5 tag Mouse Monoclonal Antibody [A10-D0-A1]

Application

  • WB

  • ELISA

  • IF-Cell

Reactivity

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

V5 tag Mouse Monoclonal Antibody [A10-D0-A1]

抗体类型

Mouse Monoclonal Antibody

免疫原

Peptide GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.

种属反应性

Species independent

验证应用

WB, ELISA, IF-Cell

阳性对照

V5-tag recombinant protein.

偶联

unconjugated

克隆号

A10-D0-A1

RRID

AB_3073026

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein G affinity purified.

应用稀释度

  • WB

  • 1:5,000-1:10,000

  • ELISA

  • 1:20,000-1:50,000

  • IF-Cell

  • 1:1,000

发表文章中的应用

WB See 4 publications below

发表文章中的种属

None See 2 publications below
Human See 1 publications below
Escherichia coli See 1 publications below

靶点

功能

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The anti-V5 antibody detects recombinant proteins containing the 14 amino acid GKPIPNPLLGLDST sequence.

背景文献

暂无

别名

GKPIPNPLLGLDST epitope tag antibody

GKPIPNPLLGLDST tag antibody

Protein Rev antibody

Regulator of expression of viral proteins antibody

rev antibody

V5 epitope tag antibody

图片

  • Western blot analysis of V5 tag on different lysates with Mouse anti-V5 tag antibody (<a href="/products/M1008-2" style="font-weight: bold;text-decoration: underline;">M1008-2</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate<br />Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1008-2" style="font-weight: bold;text-decoration: underline;">M1008-2</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of V5 tag on different lysates with Mouse anti-V5 tag antibody (M1008-2) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
    Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-2) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling V5 tag with Mouse anti-V5 tag antibody (<a href="/products/M1008-2" style="font-weight: bold;text-decoration: underline;">M1008-2</a>) at 1/1,000 dilution.<br /><br />HeLa cells, transfected with V5-tagged empty control, Histone H3 (N-terminal) or Nanos homolog 3 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-V5 tag antibody (<a href="/products/M1008-2" style="font-weight: bold;text-decoration: underline;">M1008-2</a>) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />V5 tag (<a href="/products/ET1605-41" style="font-weight: bold;text-decoration: underline;">ET1605-41</a>, green) was stained at 1/2,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling V5 tag with Mouse anti-V5 tag antibody (M1008-2) at 1/1,000 dilution.

    HeLa cells, transfected with V5-tagged empty control, Histone H3 (N-terminal) or Nanos homolog 3 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-V5 tag antibody (M1008-2) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    V5 tag (ET1605-41, green) was stained at 1/2,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  1. WB