TLE 1 Recombinant Rabbit Monoclonal Antibody [JE41-40]
Rmb: 699 特惠 1500
Catalog# HA722702
TLE 1 Recombinant Rabbit Monoclonal Antibody [JE41-40]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
TLE 1 Recombinant Rabbit Monoclonal Antibody [JE41-40]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human TLE 1 aa 201-250 / 770.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
靶点分子量
Predicted band size: 83 kDa
阳性对照
293T cell lysate, HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, Human lung tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate, 293T, human colon tissue, mouse lung tissue, rat lung tissue.
偶联
unconjugated
克隆号
JE41-40
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IF-Cell
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1:50
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IHC-P
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1:50
靶点
功能
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
背景文献
1. El Beaino M et al. Diagnostic Value of TLE1 in Synovial Sarcoma: A Systematic Review and Meta-Analysis. Sarcoma. 2020 Jan
2. Pukhalskaya T et al. TLE1 expression fails to distinguish between synovial sarcoma, atypical fibroxanthoma, and dermatofibrosarcoma protuberans. J Cutan Pathol. 2020 Feb
亚细胞定位
Nucleus.
别名
E(Sp1) homolog antibody
Enhancer of split groucho 1 antibody
Enhancer of split groucho like protein 1 antibody
Enhancer of split groucho-like protein 1 antibody
ESG antibody
ESG1 antibody
GRG1 antibody
TLE1 antibody
TLE1_HUMAN antibody
Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
展开图片
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Western blot analysis of TLE 1 on different lysates with Rabbit anti-TLE 1 antibody (HA722702) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: Human lung tissue lysate (40 µg/Lane)
Lane 6: Mouse lung tissue lysate (40 µg/Lane)
Lane 7: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722702) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of 293T cells labeling TLE 1 with Rabbit anti-TLE 1 antibody (HA722702) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TLE 1 antibody (HA722702) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TLE 1 antibody (HA722702) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722702) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-TLE 1 antibody (HA722702) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722702) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-TLE 1 antibody (HA722702) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722702) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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