Skip to content

T7 tag Mouse Monoclonal Antibody [4-C2]

RMB: 599 特惠 1500

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# EM50802

T7 tag Mouse Monoclonal Antibody [4-C2]

Application

  • WB

  • ELISA

  • IP

  • IF-Cell

Reactivity

  • Species independent

概述

产品名称

T7 tag Mouse Monoclonal Antibody [4-C2]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide CMASMTGGQQMG.

种属反应性

Species independent

验证应用

WB, ELISA, IP, IF-Cell

阳性对照

T7-tagged recombinant protein.

偶联

unconjugated

克隆号

4-C2

RRID

AB_3069005

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • ELISA

  • Use at an assay dependent concentration.

  • IF-Cell

  • Use at an assay dependent concentration.

  • IP

  • Use at an assay dependent concentration.

靶点

功能

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.

背景文献

1. Horn AV et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013)

2. Kubota T., et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem. 283:25660-70 (2008).

别名

10 antibody

Major capsid protein 10A antibody

Major capsid protein antibody

Minor capsid protein 10B antibody

T7 epitope tag antibody

图片

  • Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (<a href="/products/EM50802" style="font-weight: bold;text-decoration: underline;">EM50802</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM50802" style="font-weight: bold;text-decoration: underline;">EM50802</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (EM50802) at 1/1,000 dilution.

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"