T7 tag Mouse Monoclonal Antibody [4-C2]
Catalog# EM50802
T7 tag Mouse Monoclonal Antibody [4-C2]
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WB
-
ELISA
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IP
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IF-Cell
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Species independent
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unconjugated
概述
产品名称
T7 tag Mouse Monoclonal Antibody [4-C2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide CMASMTGGQQMG.
种属反应性
Species independent
验证应用
WB, ELISA, IP, IF-Cell
阳性对照
T7-tagged recombinant protein.
偶联
unconjugated
克隆号
4-C2
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
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ELISA
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Use at an assay dependent concentration.
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IF-Cell
-
Use at an assay dependent concentration.
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IP
-
Use at an assay dependent concentration.
靶点
功能
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.
背景文献
1. Horn AV et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013)
2. Kubota T., et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem. 283:25660-70 (2008).
别名
10 antibody
Major capsid protein 10A antibody
Major capsid protein antibody
Minor capsid protein 10B antibody
T7 epitope tag antibody
图片
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Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (EM50802) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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