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RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

RMB: 599 特惠 1500

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Catalog# ET1704-21

RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

Application

  • WB

Reactivity

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1-216 / 231.

种属反应性

Species independent

验证应用

WB

分子量

Predicted band size: 26 kDa

阳性对照

RFP recombinant protein.

偶联

unconjugated

克隆号

JA10-52

RRID

AB_3070472

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

发表文章中的应用

WB See 1 publications below

发表文章中的种属

Grass carp See 1 publications below
Mouse See 1 publications below

靶点

功能

Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).

背景文献

1. Szymanska E et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016).

2. McWilliams TG et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214:333-45 (2016).

序列相似性

Belongs to the GFP family.

翻译后修饰

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.

UNIPROT #

SwissProt: Q8ISF8 EntacmaeaQuadricolorEqFP611

别名

DsRed antibody

GFP like chromoprotein antibody

red fluorescent protein antibody

RFP antibody

图片

  • Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-21" style="font-weight: bold;text-decoration: underline;">ET1704-21</a>) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-21) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB