RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
Catalog# HA750408
RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
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WB
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Species independent
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ET1704-21
含抗保成分
-
unconjugated
概述
产品名称
RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1-216 / 231.
种属反应性
Species independent
验证应用
WB
分子量
Predicted band size: 26 kDa
阳性对照
RFP recombinant protein.
偶联
unconjugated
克隆号
JA10-52
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
靶点
功能
Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
背景文献
1. Szymanska E et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016).
2. McWilliams TG et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214:333-45 (2016).
序列相似性
Belongs to the GFP family.
翻译后修饰
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.
别名
DsRed antibody
GFP like chromoprotein antibody
red fluorescent protein antibody
RFP antibody
图片
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Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750408) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"