ALDH2 Rabbit Polyclonal Antibody
Catalog# R1407-3
ALDH2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
ALDH2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human ALDH2 aa 18-80.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 56 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat lung tissue lysate, Hela, HepG2.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:200
靶点
功能
Aldehyde dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH2 gene located on chromosome 12. This protein belongs to the aldehyde dehydrogenase family of enzymes. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Mitochondrial aldehyde dehydrogenase belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Additionally, ALDH2 functions as a protector against oxidative stress.
背景文献
1. "Mitochondrial aldehyde dehydrogenase polymorphism in Asian and American Indian populations: detection of new ALDH2 alleles." Novoradovsky A., Tsai S.J., Goldfarb L., Peterson R., Long J.C., Goldman D. Alcohol. Clin. Exp. Res. 19:1105-1110(1995)
2. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome." Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H. J. Proteomics 96:253-262(2014)
序列相似性
Belongs to the aldehyde dehydrogenase family.
亚细胞定位
Mitochondrion matrix.
别名
Acetaldehyde dehydrogenase 2 antibody
Aldehyde dehydrogenase 2 family (mitochondrial) antibody
Aldehyde dehydrogenase 2 family antibody
Aldehyde dehydrogenase mitochondrial antibody
Aldehyde dehydrogenase, mitochondrial antibody
ALDH 2 antibody
ALDH class 2 antibody
ALDH E2 antibody
ALDH-E2 antibody
Aldh2 antibody
展开图片
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Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (R1407-3) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: SK-Br-3 cell lysate (15 µg/Lane)
Lane 4: THP-1 cell lysate (15 µg/Lane)
Lane 5: Human liver tissue lysate (30 µg/Lane)
Lane 6: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ALDH2 Recombinant Mouse Monoclonal Antibody [E4-D10-R]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
ALDH2 Recombinant Mouse Monoclonal Antibody [7-D8-R]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
同靶点&同通路的产品
ALDH2 Mouse Monoclonal Antibody [E4-D10]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
