Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19] - BSA and Azide free

Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA751400) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: MEF cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Daudi cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)

Predicted band size: 53 kDa
Observed band size: 18 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751400) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751400 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751400 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA751400 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751400 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801