Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]
概述
产品名称
Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Presenilin 1 aa 311-360.
产品特异性
Presenilin 1 / PS-1 [PSH11-19] Rabbit mAb detects endogenous levels of the CTF (carboxy-terminal fragment) of presenilin 1 (22 kDa).
种属反应性
Human, Mouse, Rat
验证应用
WB, IP
分子量
Predicted band size: 53 kDa
阳性对照
HeLa cell lysate, HEK-293 cell lysate, MEF cell lysate, HepG2 cell lysate, HL-60 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
偶联
unconjugated
克隆号
PSH11-19
反应性数据
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
靶点
功能
Presenilin-1 (PS-1) is a presenilin protein that in humans is encoded by the PSEN1 gene. Presenilin-1 is one of the four core proteins in the gamma secretase complex, which is considered to play an important role in generation of amyloid beta (Aβ) from amyloid-beta precursor protein (APP). Accumulation of amyloid beta is associated with the onset of Alzheimer's disease. Presenilins are postulated to regulate APP processing through their effects on gamma secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma secretase activity or themselves are protease enzymes. Multiple alternatively spliced transcript variants have been identified for this gene, the full-length natures of only some have been determined.
背景文献
1. Yang Y et al. Presenilin-1 (PSEN1) Mutations: Clinical Phenotypes beyond Alzheimer\'s Disease. Int J Mol Sci. 2023 May
2. Gamez-Belmonte R et al. Epithelial presenilin-1 drives colorectal tumour growth by controlling EGFR-COX2 signalling. Gut. 2023 Jun
亚细胞定位
Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, neuron projection, axon, Synapse.
别名
AD3 antibody
Ad3h antibody
FAD antibody
Homo Sapiens Clone CC44 Senilin 1 antibody
Presenilin-1 CTF12 antibody
Protein S182 antibody
PS 1 antibody
PS-1 antibody
PS1-CTF12 antibody
PSEN1 antibody
展开图片
-
Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA723301) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: MEF cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Daudi cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 18 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723301 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723301 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723301 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723301 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Targeting SHMT2-mediated membrane phospholipid remodeling for enhanced anti-GCSCs treatment
期刊: Interdisciplinary Medicine
DOI: 10.1002/INMD.20240106
IF: 13.6
应用: WB
反应种属: Human
发表时间: 2025 Apr
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Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH08-49] - BSA and Azide free
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