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Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

RMB: 699 特惠 1500

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Catalog# HA723301

Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human Presenilin 1 aa 311-360.

产品特异性

Presenilin 1 / PS-1 [PSH11-19] Rabbit mAb detects endogenous levels of the CTF (carboxy-terminal fragment) of presenilin 1 (22 kDa).

种属反应性

Human, Mouse, Rat

验证应用

WB, IP

分子量

Predicted band size: 53 kDa

阳性对照

HeLa cell lysate, HEK-293 cell lysate, MEF cell lysate, HepG2 cell lysate, HL-60 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.

偶联

unconjugated

克隆号

PSH11-19

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

靶点

功能

Presenilin-1 (PS-1) is a presenilin protein that in humans is encoded by the PSEN1 gene. Presenilin-1 is one of the four core proteins in the gamma secretase complex, which is considered to play an important role in generation of amyloid beta (Aβ) from amyloid-beta precursor protein (APP). Accumulation of amyloid beta is associated with the onset of Alzheimer's disease. Presenilins are postulated to regulate APP processing through their effects on gamma secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma secretase activity or themselves are protease enzymes. Multiple alternatively spliced transcript variants have been identified for this gene, the full-length natures of only some have been determined.

背景文献

1. Yang Y et al. Presenilin-1 (PSEN1) Mutations: Clinical Phenotypes beyond Alzheimer\'s Disease. Int J Mol Sci. 2023 May

2. Gamez-Belmonte R et al. Epithelial presenilin-1 drives colorectal tumour growth by controlling EGFR-COX2 signalling. Gut. 2023 Jun

亚细胞定位

Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, neuron projection, axon, Synapse.

UNIPROT

SwissProt: P49768 Human

SwissProt: P49769 Mouse

SwissProt: P97887 Rat

别名

AD3 antibody

Ad3h antibody

FAD antibody

Homo Sapiens Clone CC44 Senilin 1 antibody

Presenilin-1 CTF12 antibody

Protein S182 antibody

PS 1 antibody

PS-1 antibody

PS1-CTF12 antibody

PSEN1 antibody

展开

图片

  • Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (<a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: MEF cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: HL-60 cell lysate (20 µg/Lane)<br />Lane 6: Daudi cell lysate (20 µg/Lane)<br />Lane 7: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 8: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 10: Rat brain tissue lysate (40 µg/Lane)<br />Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA723301) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: MEF cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: HL-60 cell lysate (20 µg/Lane)
    Lane 6: Daudi cell lysate (20 µg/Lane)
    Lane 7: Neuro-2a cell lysate (20 µg/Lane)
    Lane 8: Mouse brain tissue lysate (40 µg/Lane)
    Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 10: Rat brain tissue lysate (40 µg/Lane)
    Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 18 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: K1801

    Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723301 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723301 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA723301 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA723301 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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