NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43]
概述
产品名称
NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human NLRP3 aa 1-250.
种属反应性
Human, Mouse
验证应用
WB
分子量
Predicted band size: 118 kDa
阳性对照
THP-1 cell lysate, THP-1 treated with 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate.
偶联
unconjugated
克隆号
PSH19-43
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
靶点
功能
NLR family pyrin domain containing 3 (NLRP3) (previously known as NACHT, LRR, and PYD domains-containing protein 3 [NALP3] and cryopyrin), is a protein that in humans is encoded by the NLRP3 gene located on the long arm of chromosome 1. NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases.
背景文献
1. Yu T et al. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. Cell Rep. 2024 Apr
2. Tengesdal IW et al. NLRP3 and cancer: Pathogenesis and therapeutic opportunities. Pharmacol Ther. 2023 Nov
亚细胞定位
Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.
别名
AGTAVPRL antibody
AII/AVP antibody
Angiotensin/vasopressin receptor AII/AVP like antibody
Angiotensin/vasopressin receptor AII/AVP-like antibody
C1orf7 antibody
Caterpiller protein 1.1 antibody
CIAS 1 antibody
CIAS1 antibody
CLR1.1 antibody
Cold autoinflammatory syndrome 1 antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA724075) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate
Lane 3: Jurkat cell lysate (low expression)
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724075) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA724075) at 1/5,000 dilution.
Lane 1: J774A.1 cell lysate
Lane 2: NIH/3T3 cell lysate (low expression)
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724075) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Tanshinone IIA suppresses endoplasmic reticulum stress-mediated IRE1α/TXNIP/NLRP3 pathway to mitigate pyroptosis in type 2 diabetic osteoporosis
期刊: Biochemical Pharmacology
DOI: 10.1016/j.bcp.2025.117356
IF: 5.6
应用: WB
反应种属: Mouse
发表时间: 2025 Sept
-
Injectable pH-Responsive Hydrogel Adapted to Gingival Crevicular Fluid Microenvironment for Periodontitis Therapy
期刊: ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.5c02776
IF: 8.3
应用: WB,IF,IHC
反应种属: Mouse
发表时间: 2025 May
-
Ursolic and oleanolic acids suppress MNNG induced malignant transformation of human gastric mucosal epithelium by regulating the PI3 K/AKT pathway
期刊: Scientific Reports
DOI: 10.1038/s41598-025-03606-3
IF: 3.9
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
Aldometanib mitigates LPS-induced Lung fibroblasts injury through the activation of AMPK-mediated signaling pathways
期刊: Toxicon
DOI: 10.1016/j.toxicon.2025.108489
IF: 2.4
应用: WB
反应种属: Human
发表时间: 2025 Jul
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Reactivity: Human,Mouse
Conjugate: unconjugated
NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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