Skip to content

NF-kB p65 Rabbit Polyclonal Antibody

Rmb: 1500

产品规格

点击询价

联系我们

邮箱: market@huabio.cn

技术支持电话：0571-86052305

0571-89986345

市场客服电话：0571-88062880

查找各地代理商

Catalog# ER0815

NF-kB p65 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

NF-kB p65 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within N-terminal human RELA.

种属反应性

Human, Mouse, Rat, Zebrafish

验证应用

WB, IHC-P, FC

靶点分子量

Predicted band size: 60 kDa

阳性对照

Human lung cancer tissue, human lung tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue, zebrafish, HepG2 cell lysate, HEK-293 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.

偶联

unconjugated

RRID

AB_3069017

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

靶点

功能

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.

背景文献

1. "Duration of nuclear NF-kappaB action regulated by reversible acetylation." Chen L.F., Fischle W., Verdin E., Greene W.C.Science 293:1653-1657(2001)

2. "A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis." Higashitsuji H., Higashitsuji H., Nagao T., Nonoguchi K., Fujii S., Itoh K., Fujita J. Cancer Cell 2:335-346(2002)

3. "Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis." Liu Y., Smith P.W., Jones D.R. Mol. Cell. Biol. 26:8683-8696(2006)

4. "SIRT2 regulates NF-kappaB dependent gene expression through deacetylation of p65 Lys310." Rothgiesser K.M., Erener S., Waibel S., Luscher B., Hottiger M.O. J. Cell Sci. 123:4251-4258(2010)

翻译后修饰

Ubiquitinated by RNF182, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.; Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes (By similarity).; Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity. Phosphorylation at Ser-276 by RPS6KA4 and RPS6KA5 promotes its transactivation and transcriptional activities.; Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3 and SIRT2. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation at Lys-310 promotes interaction with BRD4. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of Lys-310. Lys-310 is deacetylated by SIRT2.; S-nitrosylation of Cys-38 inactivates the enzyme activity.; Sulfhydration at Cys-38 mediates the anti-apoptotic activity by promoting the interaction with RPS3 and activating the transcription factor activity.; Sumoylation by PIAS3 negatively regulates DNA-bound activated NF-kappa-B.; Proteolytically cleaved within a conserved N-terminus region required for base-specific contact with DNA in a CPEN1-mediated manner, and hence inhibits NF-kappa-B transcriptional activity.

亚细胞定位

Nucleus, cytoplasm.

UNIPROT

SwissProt: Q04206 Human

SwissProt: Q04207 Mouse

Entrez Gene: 309165 Rat

别名

Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody

MGC131774 antibody

NF kappa B p65delta3 antibody

NFKB3 antibody

Nuclear Factor NF Kappa B p65 Subunit antibody

Nuclear factor NF-kappa-B p65 subunit antibody

Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody

OTTHUMP00000233473 antibody

OTTHUMP00000233474 antibody

展开

图片

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded zebrafish with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded zebrafish with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (<a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: A431 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 2 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER0815" style="font-weight: bold;text-decoration: underline;">ER0815</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 60 kDa<br />Observed band size: 65 kDa

    Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 2 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER0815, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 60 kDa
    Observed band size: 65 kDa

引文

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: unconjugated

同靶点 & 同通路的产品

metafield image

Phospho-NF-kB p65 (S536) Recombinant Rabbit Monoclonal Antibody [PSH10-58] - BSA and Azide free

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-NF-kB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00] - BSA and Azide free

Application: WB,IHC-P,FC,IP

Reactivity: Human

Conjugate: unconjugated

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: unconjugated

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27] - BSA and Azide free

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

Phospho-NF-kB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]

Application: WB,IHC-P,FC,IP

Reactivity: Human

Conjugate: unconjugated

metafield image

NF-kB p65 Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Cell,FC,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: unconjugated

metafield image

Phospho-NF-kB p65 (S536) Recombinant Rabbit Monoclonal Antibody [PSH10-58]

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Copyright @ 2026 HUABIO

网站备案号：  浙ICP备09005892号-1 |  浙公网安备 33019202000643号

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

HI！我是 LUMI，可以帮您解决产品和实验问题哦～