NCAM1 / CD56 Recombinant Rabbit Monoclonal Antibody [JF1021]
Catalog# IRS026
NCAM1 / CD56 Recombinant Rabbit Monoclonal Antibody [JF1021]
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mIHC
-
Human
概述
产品名称
NCAM1 / CD56 Recombinant Rabbit Monoclonal Antibody [JF1021]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within C-terminal human NCAM1.
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 95 kDa
阳性对照
Human tonsils tissue.
偶联
unconjugated
克隆号
JF1021
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
Neural cell adhesion molecules (NCAMs) are a family of closely related cell surface glycoproteins involved in cell to cell interactions during growth and thought to play an important role in embryogenesis and development. The expression of these molecules is widespread in all three germ layers during embryogenesis, but is more restrictive in adult tissues. NCAM expression is observed in a variety of human tumors including neuroblastomas, rhabdo-myosarcomas, Wilms' tumor, Ewing's sarcoma and some primitive myeloid malignancies. Multiple isoforms of NCAM have been reported in both mouse and human brain tissue. In humans, NCAMs arise from differential splicing and use of alternative polyadenylation sites of a single gene mapping to 11q23.
背景文献
1. Kong Q et al. Effects of pharmacological treatments on hippocampal NCAM1 and ERK2 expression in epileptic rats with cognitive dysfunction. Oncol Lett 12:1783-1791 (2016).
2. Berardis S et al. Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells. PLoS One 9:e86137 (2014).
亚细胞定位
Cell membrane, Secreted.
UNIPROT #
别名
antigen MSK39 identified by monoclonal antibody
5.1H11 antibody
antigen recognized by monoclonal antibody
5.1H11 antibody
CD56 antibody
cell adhesion molecule, neural, 1 antibody
MSK 39 antibody
MSK39 antibody
N-CAM-1 antibody
NCAM 1 antibody
展开图片
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mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-NCAM1 / CD56 antibody (IRS026) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (IRS026), CD68 (IRS004), CD163 (IRS002), panCK (IRS010) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"